A Neuronal Culture System to Detect Prion Synaptotoxicity

A Neuronal Culture System to Detect Prion Synaptotoxicity

Synaptic pathology is an early feature of prion as well as other neurodegenerative diseases. Although the self-templating process by which prions propagate is well established, the mechanisms by which prions cause synaptotoxicity are poorly understood, due largely to the absence of experimentally tractable cell culture models. Here, we report that exposure of cultured hippocampal neurons to PrP...

Amyloid-β nanotubes are associated with prion protein-dependent synaptotoxicity

Growing evidence suggests water-soluble, non-fibrillar forms of amyloid-β protein (Aβ) have important roles in Alzheimer's disease with toxicities mimicked by synthetic Aβ(1-42). However, no defined toxic structures acting via specific receptors have been identified and roles of proposed receptors, such as prion protein (PrP), remain controversial. Here we quantify binding to PrP of Aβ(1-42) af...

Amyloid-b nanotubes are associated with prion protein-dependent synaptotoxicity

Growing evidence suggests water-soluble, non-fibrillar forms of amyloid-b protein (Ab) have important roles in Alzheimer’s disease with toxicities mimicked by synthetic Ab1–42. However, no defined toxic structures acting via specific receptors have been identified and roles of proposed receptors, such as prion protein (PrP), remain controversial. Here we quantify binding to PrP of Ab1–42 after ...

A Business Model to Detect Disease Outbreaks

Introduction: Every year several disease outbreaks, such as influenza-like illnesses (ILI) and other contagious illnesses, impose various costs to public and non-government agencies. Most of these expenses are due to not being ready to handle such disease outbreaks. An appropriate preparation will reduce the expenses. A system that is able to recognize these outbreaks can earn ...

Neuronal Autophagy and Prion Proteins